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Q1(b):
Differentiate between (b) Glycolysis and Krebs’ cycle

Solution :

Definition and Theoretical Basis

Glycolysis (the Embden-Meyerhof-Parnas pathway) is the universal initial phase of cellular respiration occurring in the cytosol, wherein glucose is partially oxidized to pyruvate. The Krebs’ cycle (Citric Acid Cycle) is the secondary aerobic phase occurring within the mitochondrial matrix, involving the complete oxidation of acetyl-CoA derivatives to $CO_2$ and high-energy electron carriers.

Comparative Analytical Table

Feature Glycolysis Krebs’ Cycle
Site of Occurrence Cytosol [Cytoplasmic compartment] Mitochondrial Matrix [Inner mitochondrial compartment]
Requirement of $O_2$ Anaerobic (Does not require oxygen) Aerobic (Requires $O_2$ indirectly via ETS)
Substrate Glucose ($C_6H_{12}O_6$) Acetyl-CoA ($C_2$ unit)
Net Energy Yield $2$ $ATP$ and $2$ $NADH$ per glucose $2$ $GTP$ ($ATP$), $6$ $NADH$, $2$ $FADH_2$ per glucose
Decarboxylation Absent Present (Release of $2$ $CO_2$ per acetyl-CoA)

Sequential Metabolic Logic

1. Glycolysis Dynamics: The process involves a $10$-step enzymatic conversion of one molecule of $6$-carbon glucose into two molecules of $3$-carbon pyruvate. [By the Phosphofructokinase rate-limiting step]. Energy is harvested via substrate-level phosphorylation.

2. Krebs’ Cycle Dynamics: The cycle commences with the condensation of a $2$-carbon acetyl group with $4$-carbon oxaloacetic acid to form a $6$-carbon citrate. [Per the Citrate Synthase catalysis]. It undergoes a series of redox reactions, releasing carbon as $CO_2$, and transferring electrons to $NAD^+$ and $FAD$.

Visual Representation of Pathways

Glycolysis (Cytosol) Glucose -> Pyruvate Net: 2 ATP, 2 NADH Krebs Cycle (Matrix) Acetyl-CoA -> CO2 Net: 6 NADH, 2 FADH2 Link Reaction

Final Solution: Glycolysis is the anaerobic breakdown of glucose in the cytosol resulting in pyruvate and minimal ATP, whereas the Krebs’ cycle is the aerobic completion of glucose oxidation within the mitochondria, yielding primary electron carriers ($NADH, FADH_2$) for oxidative phosphorylation.


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