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Q16(ii):
Analyse the events during every stage of cell cycle and notice how the following two parameters change (ii) amount of DNA content ($C$) per cell

Solution :

Initial Parameters & Definitions

The cell cycle is a regulated sequence of events leading to cellular replication. The parameter $C$ represents the amount of DNA content in a haploid genome. In a diploid somatic cell ($2n$), the initial DNA content is defined as $2C$. We track the variations of $C$ across the distinct phases: $G_1$, $S$, $G_2$, and $M$ phases.

Step 1: The Gap 1 ($G_1$) Phase

During the $G_1$ phase, the cell undergoes metabolic growth and prepares for DNA replication. As the cell has not yet initiated the synthesis of new genetic material:

  • DNA content: Remains $2C$.
  • [Per the definition of the post-mitotic phase in cell cycle regulation].

Step 2: The Synthesis ($S$) Phase

During the $S$ phase, the process of DNA replication occurs. The cell synthesizes an exact copy of each chromosome.

  • Process: The DNA content doubles as each chromatid is replicated.
  • DNA content transition: Increases linearly from $2C$ to $4C$.
  • [Per the semi-conservative replication mechanism of DNA].

Step 3: The Gap 2 ($G_2$) Phase

Following replication, the cell enters $G_2$ where it prepares for mitosis by synthesizing proteins and organelles.

  • DNA content: Remains stable at $4C$.
  • [Per the state of post-replicative stability].

Step 4: The Mitotic (M) Phase

The M-phase involves the separation of replicated chromosomes (sister chromatids) into two daughter nuclei.

  • Prophase to Anaphase: The DNA content remains at $4C$.
  • Anaphase: Sister chromatids separate, and the cell is considered to possess two sets of chromosomes.
  • Telophase/Cytokinesis: The cell physically divides, splitting the $4C$ content equally into two daughter cells.
  • Final DNA content per daughter cell: $2C$.
  • [Per the Law of Segregation and the mechanics of the mitotic spindle].

Graphical Representation of DNA Content ($C$) Over Time

DNA (C) Cell Cycle Phases G1 S G2 M 2C 4C

Summary Table of DNA Content

Phase DNA Content
$G_1$ $2C$
$S$ $2C \rightarrow 4C$
$G_2$ $4C$
Mitosis (until Anaphase) $4C$
Post-Cytokinesis $2C$ (per daughter cell)

Final Solution: The DNA content per cell remains $2C$ during $G_1$, increases to $4C$ during the $S$ phase, remains $4C$ through $G_2$ and the majority of mitosis, and returns to $2C$ in each daughter cell upon the completion of cytokinesis.


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