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Sangeeta S.

Ph.D Zoology ( Molecular biology)

Dapodi, Pune, India- 411012.

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Overview

Experience of 3 years teaching to FY and SY B.Sc. students

Demo Class

No

Address

Flat no. A/12, Ganesh Garden Phase 3

Ganesh Nagar, Dapodi

Dapodi, Pune, India- 411012.

BSc Tuition Overview

BSc Tuition

Class Location

Student's Home

Tutor's Home

Online (video chat via skype, google hangout etc)

Years of Experience in BSc Tuition

4

View all Classes

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Answers by Sangeeta S. (3)

"What is melting temperature of DNA? Define denaturation and renaturation?" in   Tuition/BSc Tuition

The melting temperature (Tm) of the DNA means "the temperature at which the half of the Double Stranded DNA becomes Single Strand DNA" means this is the temperature at which the DNA is denaturating or melting from its Native form, hence we call that temperature as melting temperature of the DNA. Now if DNA contains more GC base pairs( more hydrogen bonds comparitively than AT) then it requires more energy (temperature) for breaking the bonds. Here in this case the Tm of Primere varies depends on the composition. formula for predicting the Tm of the primer (4(G+C) + 2(A+T))-5. DNA denaturation refers to the unwinding and separation (melting) of the two strands of a double-helical DNA molecule.In DNA renaturation, double helical molecules are formed by the annealing of single-stranded molecules. DNA

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"What is the full name of IUPAC?" in   Tuition/BSc Tuition

International Union of Pure and Applied Chemists, the standards body that among other things, makes recommendations regarding the names of newly discovered elements and forms other chemistry related standards (such as the labeling of groups on the periodic table).

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"How to identify an unknown sample whether it is a DNA sample or RNA sample? And by which method can you determine its concentration?" in   Tuition/BSc Tuition

Three Different Ways: 1. The simplest method is to go with UV spec - Measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm. Pure sample of DNA has the 260/280 ratio at 1.8. DNA contaminated with protein will have a 260/280 ratio lower than 1.8. A sample containing only RNA following an extraction method is judged as being uncontaminated if the ratio is 1 :2 : 1, and for DNA is 1 : 1.8 : 1 (it reflects OD-230 : 260 : 280 ratio). If there is significant deviation from the ratio, then it is evident that contaminants are present and that further purification of the sample is necessary. 2. The other two can be identified in this way: as we know that DNA has deoxy sugar, i.e one oxygen is missing as compared to RNA having ribose sugar, so this RNA is ressistant to alkaline hydrolyiss whereas DNA is not. 3. If you shake the test tube vigorously proteins will give froth, Where in case of DNA solution you are suppose to view swirling motion of DNA but in case of RNA you can't able to see any froth or swirl like things. 4. Agarose gel electrophoresis is another way to quickly estimate DNA concentration. The percentage of agarose in the gel will determine what size range of DNA will be resolved with the greatest clarity. Any RNA, nucleotides and protein in the sample migrate at different rates compared to the DNA so the band(s) containing the DNA will be distinct.

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Sangeeta S. address

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BSc Tuition

Class Location

Student's Home

Tutor's Home

Online (video chat via skype, google hangout etc)

Years of Experience in BSc Tuition

4

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No Reviews yet! Be the first one to Review

"What is melting temperature of DNA? Define denaturation and renaturation?" in   Tuition/BSc Tuition

The melting temperature (Tm) of the DNA means "the temperature at which the half of the Double Stranded DNA becomes Single Strand DNA" means this is the temperature at which the DNA is denaturating or melting from its Native form, hence we call that temperature as melting temperature of the DNA. Now if DNA contains more GC base pairs( more hydrogen bonds comparitively than AT) then it requires more energy (temperature) for breaking the bonds. Here in this case the Tm of Primere varies depends on the composition. formula for predicting the Tm of the primer (4(G+C) + 2(A+T))-5. DNA denaturation refers to the unwinding and separation (melting) of the two strands of a double-helical DNA molecule.In DNA renaturation, double helical molecules are formed by the annealing of single-stranded molecules. DNA

0
|
0

"What is the full name of IUPAC?" in   Tuition/BSc Tuition

International Union of Pure and Applied Chemists, the standards body that among other things, makes recommendations regarding the names of newly discovered elements and forms other chemistry related standards (such as the labeling of groups on the periodic table).

0
|
0

"How to identify an unknown sample whether it is a DNA sample or RNA sample? And by which method can you determine its concentration?" in   Tuition/BSc Tuition

Three Different Ways: 1. The simplest method is to go with UV spec - Measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm. Pure sample of DNA has the 260/280 ratio at 1.8. DNA contaminated with protein will have a 260/280 ratio lower than 1.8. A sample containing only RNA following an extraction method is judged as being uncontaminated if the ratio is 1 :2 : 1, and for DNA is 1 : 1.8 : 1 (it reflects OD-230 : 260 : 280 ratio). If there is significant deviation from the ratio, then it is evident that contaminants are present and that further purification of the sample is necessary. 2. The other two can be identified in this way: as we know that DNA has deoxy sugar, i.e one oxygen is missing as compared to RNA having ribose sugar, so this RNA is ressistant to alkaline hydrolyiss whereas DNA is not. 3. If you shake the test tube vigorously proteins will give froth, Where in case of DNA solution you are suppose to view swirling motion of DNA but in case of RNA you can't able to see any froth or swirl like things. 4. Agarose gel electrophoresis is another way to quickly estimate DNA concentration. The percentage of agarose in the gel will determine what size range of DNA will be resolved with the greatest clarity. Any RNA, nucleotides and protein in the sample migrate at different rates compared to the DNA so the band(s) containing the DNA will be distinct.

0
|
0

Sangeeta S. conducts classes in BSc Tuition. Sangeeta is located in Dapodi, Pune. Sangeeta takes at students Home, Regular Classes- at her Home and Online Classes- via online medium. She has 4 years of teaching experience .

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