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Q8:
Try to collect all the currently accepted meanings for the word ‘species’. Discuss with your teacher the meaning of species in case of higher plants and animals on one hand, and bacteria on the other hand.

Solution :

1. Definitions of Species: Multi-Dimensional Perspectives

The term "species" serves as the fundamental unit of biological classification, yet its definition varies based on the organism's mode of reproduction and evolutionary history.

Concept Definition Primary Criterion
Biological Species Concept (BSC) Groups of interbreeding natural populations that are reproductively isolated from other such groups. [Proposed by Ernst Mayr, 1942]. Reproductive Isolation
Phylogenetic Species Concept The smallest diagnosable cluster of individual organisms within which there is a parental pattern of ancestry and descent. Monophyly
Ecological Species Concept A set of organisms adapted to a particular set of resources, called a niche, in the environment. Ecological Niche

2. Analysis of Species: Higher Plants and Animals vs. Bacteria

The definition of species deviates significantly when comparing eukaryotes (plants/animals) and prokaryotes (bacteria).

  • Higher Plants and Animals: These organisms predominantly reproduce sexually. The Biological Species Concept (BSC) is the standard metric. Species are defined by their ability to interbreed to produce fertile offspring. Reproductive isolation is the key mechanism maintaining the integrity of these species.
  • Bacteria: Bacteria reproduce primarily via binary fission (asexual reproduction) and engage in horizontal gene transfer (HGT) via transformation, transduction, and conjugation. Because they lack sexual reproduction, the BSC is inapplicable. Instead, microbiologists use the Polyphasic Approach.

3. Taxonomic Criteria for Bacterial Species

Due to the lack of sexual markers, bacterial species are defined using a combination of genetic and phenotypic traits:

  1. 16S rRNA Sequence Similarity: A threshold of $\ge 97\%$ (or more recently $\ge 98.7\%$) identity in the 16S rRNA gene is used as a proxy for taxonomic classification. [Per Woese & Fox, 1977].
  2. DNA-DNA Hybridization (DDH): Traditionally, if two strains show $\ge 70\%$ genomic DNA hybridization, they are considered to be in the same species.
  3. Average Nucleotide Identity (ANI): Modern genomics uses ANI, where a value of $\ge 95-96\%$ is now the accepted standard for defining a bacterial species.

4. Comparative Summary Diagram

Higher Organisms: - Sexual Reproduction - Reproductive Isolation Bacteria (Prokaryotes): - Asexual / Fission - Genomic Identity (ANI)

Final Solution: The definition of 'species' is inherently contextual; while higher organisms are defined by the ability to interbreed and maintain reproductive isolation, bacteria are defined by genomic sequence similarity and phenotypic characteristics due to their asexual nature and horizontal gene transfer capabilities.


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