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Raj replied | 01/03/2016

When DNA is heated the double strands unwind and separate into single strands through the breaking of the hydrogen bonding between the bases. The term used to refer to this process is denaturation. Denaturation can be accomplished by other methods but in molecular diagnostic testing heat is the method of choice.

The Temperature of Melting (Tm) is defined as the temperature...  more»
When DNA is heated the double strands unwind and separate into single strands through the breaking of the hydrogen bonding between the bases. The term used to refer to this process is denaturation. Denaturation can be accomplished by other methods but in molecular diagnostic testing heat is the method of choice.

The Temperature of Melting (Tm) is defined as the temperature at which 50% of double stranded DNA is changed to single-standard DNA. The higher the melting temperature the greater the guanine-cytosine (GC) content of the DNA.

The melting temperature depends on both the length of the molecule, and the specific nucleotide sequence composition of that molecule. The guanine-cytosine pair is bound by three hydrogen bonds, while adenine-thymine pairs are bound by two hydrogen bonds. DNA with high GC content is more stable than DNA with low GC content. In PCR experiments GC content of primers are used to predict their annealing temperature to the template DNA. A higher GC content level indicates a higher melting temperature.
Formula:
Tm = 2 °C(A + T) + 4 °C(G + C) = °C Tm «less

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Supriya Nath replied | 20/06/2016

Ok..the previous answers are true but without knowing the ' GC' content one can use 95 to 92 degree celsius for eukaryotic DNA. We use that temp. while performing PCR.

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Shikha replied | 06/08/2015

For identification of DNA and RNA we use UVspectrophotometer or caloriemeter

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Harina replied | 06/08/2015

Quantitative & qualitative- colorimetric (DNA by DPA method, RNa by Orcinol method )or
also by spectrophotometric method can be conducted.

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Suryakesh 06/12/2014 in  BSc Tuition

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Krishna replied | 12/07/2016

IUPAC Stands for International Union for Pure and Applied Chemistry

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Sarvajeet replied | 07/12/2016

The International Union of Pure and Applied Chemistry = IUPAC.

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Kalyani replied | 02/01/2015

Classical crystallographic restriction theorem says that crystals can posses only two, three, four and six-fold rotational symmetries but QUASI CRYSTALS OR QUASI PERIODIC CRYSTALS may show five-fold and other symmetry orders too.Quasi crystal is a structure that is ordered but not periodic.

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U.k. replied | 22/01/2015

Pentagonal symmetry is present in a molecule but not in crystal. it is true..

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Isaiah replied | 28/05/2016

RED GIANT

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Sarvajeet replied | 07/12/2016

Maxima and Minima rule use first order derivative test and second order derivative test then we can find find out values.

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