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Q2(c):
Differentiate between the following – (c) Monohybrid and Dihybrid.

Solution :

Definition and Theoretical Framework

The distinction between a monohybrid and a dihybrid cross is rooted in the number of contrasting traits under investigation during a hybridization experiment, governed by Mendel’s Laws of Inheritance.

Feature Monohybrid Cross Dihybrid Cross
Definition A cross considering a single pair of contrasting alleles. A cross considering two pairs of contrasting alleles.
Governing Law Law of Dominance and Law of Segregation. Law of Independent Assortment.
Genotypic Ratio ($F_2$) $1:2:1$ $1:2:1:2:4:2:1:2:1$
Phenotypic Ratio ($F_2$) $3:1$ $9:3:3:1$

Step 1: The Monohybrid Mechanism

A monohybrid cross involves tracking one character (e.g., plant height). [Per the Law of Segregation, alleles for a trait separate during gamete formation so that each gamete carries only one allele].

Example: $TT \times tt \rightarrow F_1: Tt \rightarrow F_2: 3 \text{ Tall} : 1 \text{ Dwarf}$.

Step 2: The Dihybrid Mechanism

A dihybrid cross involves tracking two characters (e.g., seed color and seed shape). [Per the Law of Independent Assortment, the segregation of one pair of alleles is independent of the segregation of another pair of alleles].

Example: $YYRR \times yyrr \rightarrow F_1: YyRr \rightarrow F_2: 9:3:3:1$ phenotypic ratio.

Step 3: Visual Representation of Segregation Patterns

Monohybrid (1 trait) Segregation Dihybrid (2 traits) Independent Assortment

Analytical Summary

The Monohybrid cross is used to establish the concept of dominance and the purity of gametes. The Dihybrid cross provides the proof for the independent inheritance of distinct traits, resulting in a broader genetic recombination in the $F_2$ generation.

Final Solution: A monohybrid cross analyzes the inheritance pattern of one trait governed by the Law of Segregation, yielding a $3:1$ phenotype ratio, whereas a dihybrid cross analyzes two traits governed by the Law of Independent Assortment, yielding a $9:3:3:1$ phenotype ratio.


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