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Q2(c):
Differentiate between the following – (c) Monohybrid and Dihybrid.

Solution :

Definition and Theoretical Foundation

The distinction between a monohybrid and a dihybrid cross is rooted in the number of contrasting traits under observation during a hybridization experiment, governed by Mendel’s Laws of Inheritance. [Per the Law of Segregation and the Law of Independent Assortment].

Feature Monohybrid Cross Dihybrid Cross
Definition Cross involving a single pair of contrasting characters. Cross involving two pairs of contrasting characters.
Governing Law Law of Segregation (Mendel's First Law). Law of Independent Assortment (Mendel's Second Law).
Typical Genotypic Ratio ($F_2$) $1:2:1$ $(1:2:1)^2 = 1:2:1:2:4:2:1:2:1$
Typical Phenotypic Ratio ($F_2$) $3:1$ $9:3:3:1$

Step 1: Analyzing the Monohybrid Cross

In a monohybrid cross, we track the inheritance of one trait (e.g., stem height). Parents: $TT$ (Tall) $\times$ $tt$ (Dwarf). The $F_1$ generation is $Tt$. Selfing the $F_1$ generation results in $F_2$ gametes following a $1:2:1$ genotypic distribution.

Step 2: Analyzing the Dihybrid Cross

In a dihybrid cross, two traits are studied simultaneously (e.g., seed shape and seed color). Parents: $RRYY$ (Round Yellow) $\times$ $rryy$ (Wrinkled Green). The $F_1$ generation is $RrYy$. The $F_2$ generation exhibits 16 possible combinations, resulting in the $9:3:3:1$ phenotypic ratio [By Independent Assortment of alleles on non-homologous chromosomes].

Step 3: Visual Representation

Monohybrid (1 trait) P: TT x tt F1: Tt F2 Ratio: 3:1 Dihybrid (2 traits) P: RRYY x rryy F1: RrYy F2 Ratio: 9:3:3:1

Conclusion on Biological Variation

The monohybrid cross demonstrates the purity of gametes and dominance. Conversely, the dihybrid cross demonstrates that factors for different traits segregate and assort independently, thereby increasing genetic variation within a population [Per the Law of Independent Assortment].

Final Solution: A monohybrid cross involves the inheritance pattern of one distinct trait (ratio $3:1$ phenotypic), while a dihybrid cross involves the simultaneous inheritance of two traits (ratio $9:3:3:1$ phenotypic), governed by Mendel’s Second Law of Independent Assortment.


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