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Avery-MacLeod-McCarty experiment. How pneumococcus genetically transformed from avirulent to virulent form?

Durgapada Rana
07/05/2018 0 0

In 1944, Oswald Avery, Colin MacLeod, and Maclyn McCarty revisited Griffith's experiment and concluded that the transforming material was pure DNA, not protein or RNA. These investigators found that DNA extracted from a virulent strain of the bacterium Streptococcus pneumoniae, also known as pneumococcus, genetically transformed an avirulent strain of this organism into a virulent form. 

 

Type IIR cells  ⇒ No transformation

Type IIR cells + Type IIIS DNA extract ⇒ Transformation

Type IIR cells + Type IIIS DNA extract + DNase ⇒ No transformation 

Type IIR cells + Type IIIS DNA extract + RNase ⇒ Transformation 

Type IIR cells + Type IIIS DNA extract + Protease ⇒ Transformation

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