Wadgaon Sheri, Pune 
Identity
Lab Experience :-
July05 Aug05:- One month training on fundamental techniques in Botany Dept. from Chaudhary Charan Singh University.
1st Aug06 - 31st Aug06:-One month training from DABUR (D.R.F.) worked on assigned project on (BIOASSAY OF ANTIBIOTIC).
Jan11 Jun11:- 6 month training on CLONING AND EXPRESSION OF AMYLOID BETA PEPTIDE OF ALZHEIMER'S DISEASE and using technique of ISOLATION AND PURIFICATION and other techniques used in this project from AIIMS ( All India Institute Of Medical Science )
Technical proficiency :-
Handling of Spectrophotometer, Bacterial culture, Isolation of plant and animal DNA, Isolation of DNA and RNA, primer designing Performing Polymerase Chain Reaction (PCR), Analysis of PCR product, Cloning of PCR amplified product in pGEM-T Easy, Ligation in pGEM-T Easy cloning vector,Gel Elution, Cloning of amplified product, Plasmid isolationPreparation of DH5- alpha competent cell, Transformation, Restriction digestion of isolated DNA, Subcloning in pet 22b +, Expression of Protein, Western blotting Gel electrophoresis (Agarose gel electrophoresis), Purification of recombinant, protein by Ni-NTA affinity chromatography, Regeneration of Ni-NTA Resin , DNA Quantification by Nanodrop, Preparation of different culture media.
Certified
