Rahul Kharade

Work Experience:
Company name: Bio Re Inventors Lab LLP.
ï?· Designation: Operation Manager
ï?· Duration: 10 th June 2016 to still today
ï?· Role and Responsibilities:
Skills and laboratory techniques: Basic and advanced techniques in a Microbiology
including aseptic microbiology practices ,microscopy ,sterilization techniques,
biochemical analysis ,staining, handling and maintenance of various bacteria and other
microorganisms, lab ethics
(Heading the Commercial and R&D division of BRi, Counseling the students regarding
Trainings .Has successful in collaborating with various industry for service and project.)
Company name: Grotrade
ï?· Designation: Biotechnology Research In-Charge.
ï?· Duration:20th Sep 2015 to 10th Aug, 2016
(Heading the Commercial and R&D division of Grotrade, Guiding the team
regarding Tissue culture and Horticulture .Has successful in collaborating with various industry
for service and project.)
Company name: Ventri Biologicals, Malkhed Pune.
ï?· Department: Marek Disease Vaccine production plant.
ï?· Designation: â?? Biotechnologist
ï?· Duration: 3 March 2015 to 2nd sep2015
Role and Responsibilities:
ï?· Working on Marekâ??s Disease live attenuated viral vaccine, based on cell culture.
Strains viz HVT.
ï?· Preparation of chick embryo fibroblast cell line from Specific Pathogen Free (SPF)
eggs.
ï?· Infection of chick embryo fibroblast cell line, harvesting of virus.
ï?· Final bulk preparation, packaging and cryopreservation.
ï?· Simultaneously involved in in process sterility.
ï?· Preparation of all types of media related to process. Such as Minimum Essential
Medium (MEM), Trypsin solution, Antibiotic Solution.
ï?· To execute all production activities as per Good Manufacturing Practices.
Lab Skills&Technical skill set:
ï?· Microbiology Standard techniques in microbiology of bacteria and fungi.
(Isolation, Preservation, Staining)
ï?· Biochemistry: Basic biochemical estimation technique for biomolecules (DNA, RNA,
Protein estimation, etc.), Western blotting, thin layer chromatography, UV
spectroscopy
ï?· Plant Biotechnology: Different tissue culture technique (callus, shoot tip culture)
ï?· Cell & Molecular Biology: DNA isolation, RNA isolation, Electrophoresis, Southern
blotting, Northern blotting
ï?· Immunology: Single and Double diffusion, ELISA, Rocket immune electrophoresis.
Operating System Windows 9x/2000/XP/7/8, DOS, IDAS
Course MS-CIT, TMV,Digital Marketing