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Q8(f):
Which one of the plant growth regulators would you use if you are asked to: (f) induce immediate stomatal closure in leaves.

Solution :

Identification of the Physiological Agent

To induce immediate stomatal closure in leaves, the plant growth regulator required is Abscisic Acid (ABA).

Step 1: Mechanism of Stomatal Action

Stomatal aperture regulation is primarily controlled by the turgor pressure of guard cells. When guard cells are turgid, the stomatal pore is open; when they lose water and become flaccid, the pore closes [Per the Turgor Theory of Stomatal Movement].

Step 2: Signaling Cascade of Abscisic Acid

Abscisic acid functions as a stress hormone, particularly under conditions of water deficit. The physiological induction follows this sequence:

  • Perception: ABA binds to PYR/PYL/RCAR receptors located in the plasma membrane and cytosol of guard cells.
  • Inhibition: The ABA-receptor complex inhibits Type 2C Protein Phosphatases (PP2Cs), which are negative regulators of the pathway.
  • Activation: This releases SnRK2 kinases, which phosphorylate downstream targets, including anion channels (such as SLAC1) and inward-rectifying $K^+$ channels.
  • Efflux of Ions: Anion channels open, facilitating the efflux of $Cl^-$ and $NO_3^-$. Simultaneously, the inhibition of inward-rectifying $K^+$ channels prevents $K^+$ uptake, while outward-rectifying $K^+$ channels are activated to facilitate $K^+$ efflux.

Step 3: Osmotic Consequence and Turgor Loss

The rapid efflux of ions ($K^+$, $Cl^-$, $NO_3^-$) from the guard cell cytosol into the apoplast decreases the osmotic potential of the guard cells [Per the Principle of Osmosis]. Consequently, water follows the solute gradient, exiting the cells via aquaporins. The loss of hydrostatic pressure causes the guard cells to lose turgor, leading to the physical closure of the stomatal pore [By the Law of Hydrostatics].

Visual Representation of Stomatal Flux

Turgid (Open) Flaccid (Closed) ABA Application

Comparative Summary of Ion Flux

Ion Type Direction of Movement Resulting Osmotic Potential
$K^+$ (Potassium) Efflux (Exit) Increased (Less negative)
$Cl^- / NO_3^-$ Efflux (Exit) Increased (Less negative)

Final Solution: Abscisic Acid (ABA) is the plant growth regulator used to induce immediate stomatal closure.


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