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Q8(a):
Which one of the plant growth regulators would you use if you are asked to: (a) induce rooting in a twig

Solution :

Identification of the Plant Growth Regulator

To induce rooting in a cut twig (stem cutting), the plant growth regulator of choice is Auxin, specifically Indole-3-butyric acid (IBA) or 1-Naphthaleneacetic acid (NAA).

Step 1: Theoretical Basis of Root Initiation

Rooting in vegetative propagules (cuttings) is a physiological process known as adventitious root formation. [Per the hormonal theory of organogenesis, the balance of internal auxins and cytokinins dictates cell differentiation]. When a twig is detached, the high concentration of endogenous auxins synthesized in the apical meristems is disrupted. The application of exogenous auxins artificially stimulates the pericycle cells or vascular cambium cells to undergo dedifferentiation and subsequently develop into root primordia.

Step 2: Role of Indole-3-Butyric Acid (IBA)

IBA is the synthetic or naturally occurring auxin most favored in horticultural practices for the following reasons:

  • High Physiological Activity: It exhibits strong translocation properties and higher stability than Indole-3-acetic acid (IAA).
  • Cell Differentiation Induction: It promotes the transformation of unspecialized parenchyma cells into organized root apical meristems.
  • Polar Transport: Auxins move in a polar fashion (basipetal transport), ensuring the accumulation of the hormone at the basal cut end, which triggers the emergence of roots [By the Cholodny-Went Theory of phototropism and growth distribution].

Step 3: Schematic Visualization of Root Induction

Twig Stem Induced Roots Auxin Application (IBA) at Basal Node

Step 4: Comparative Analysis of Regulatory Effectiveness

Regulator Primary Function Suitability for Rooting
Auxins (IBA/NAA) Root initiation High (Standard Choice)
Gibberellins Stem elongation Low (Can inhibit rooting)
Cytokinins Cell division/Shoot formation Low (Promotes shoots over roots)

Final Solution: The plant growth regulator to be used is an Auxin, specifically Indole-3-butyric acid (IBA), as it effectively triggers the dedifferentiation of cells at the basal end of the twig to form adventitious roots.


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