Rakhi M.

GENERAL LAB SKILLS - Follow laboratory protocol, Maintain laboratory notebook.
CHEMISTRY TECHNIQUES - Solution preparation, Molarity calculations, Serial dilutions, Sterile Media Preparation, Use of pH, Buffer & Reagent preparation. Volumetric Analysis, Colorimetry.
PROTEIN TECHNIQUES - Differential Centrifugation/Purification , Chromatography, SDS-PAGE , Molecular Weight Determination, Enzyme-Linked Immunosorbent Assay (ELISA).
DNA/RNA TECHNIQUES - Agarose Gel Electrophoresis for DNA Separation/Analysis, Plasmid/Genomic DNA Isolation, Staining/Destaining & Analysis of DNA Gels, RNA Isolation,Restriction Digestion of genomic DNA, Southern & Northern Blotting, Competent Cell Preparation & Blue- White Colony Screening, cDNA Preparation, Cloning & Digestion, PCR , Colony PCR and Primer Designing.
INSTRUMENTAL ANALYSIS TECHNIQUES - Standards Preparation of medium,Use Of Spectrophotometer, PCR Method Setup, Microscope handling, Autoclaving, Operating LAF & others.
CELL CULTURE TECHNIQUES - Isolation and Characterization of Plant Genomic DNA, Growth of Various Bacterial Strains In Liquid or Semi-Solid Media, Various Sterilization techniques.
IMMUNOTECHNOLOGY TESTS - Haemaglutination Reaction, Immunopricipitation, Agglutination, RBC Count, Haemogloubin Level, ELISA.
OPERATING SYSTEMS - Window XP, Window7,8