Kirti S.

I am a Molecular Biologist currently working as an Executive in a Cell and Gene Therapy company, with strong hands-on and theoretical experience in modern molecular biology and genetic engineering techniques. I am passionate about teaching and enjoy helping students build confidence in both foundational concepts and practical lab skills.

I can provide clear, structured, and application-based learning in the following areas:

PCR & RT-PCR – Principle, assay design, optimization, troubleshooting, and data interpretation

Gene Cloning & Molecular Techniques – Restriction digestion, ligation, transformation, plasmid preparation, vector design

Cell Culture Techniques – Aseptic handling, passaging, maintenance, viability analysis, cryopreservation (freezing & thawing)

Viral Vector Workflows – Basics of lentiviral production, viral titer estimation (qPCR/ddPCR-based), DNase treatment, and workflow optimization

Data Analysis & Troubleshooting – Ct interpretation, knockdown studies, assay comparison (SYBR vs TaqMan), dilution calculations, and experimental planning

Lab Best Practices – SOP-based learning, documentation, safety, and experimental accuracy


My teaching approach is student-friendly, focusing on:

Breaking down complex concepts into simple, easy-to-understand explanations

Providing real-lab examples, protocols, and troubleshooting strategies

Helping students prepare for lab courses, academic research, internships, and biotech industry roles

Offering personalized guidance based on your current level and goals