Find the best tutors and institutes for Class 12 Tuition

Find Best Class 12 Tuition

Please select a Category.

Please select a Locality.

No matching category found.

No matching Locality found.

Outside India?

What is a Polymerase Chain Reaction (PCR)?

Alka S.
28 May 0 0

It is a revolutionary method developed by Kary Mullis in the 1980s, used to synthesize a new strand of DNA complementary to the offered template strand. 

Components of PCR:

1. DNA template- the DNA strand that contains the target sequence which has to be multiplied.

2. DNA Polymerase enzyme- a type of enzyme that synthesizes new strands of DNA complementary to the target sequence. The first and most commonly used of these enzymes is Taq DNA polymerase (from Thermus aquaticus), whereas PfuDNA polymerase (from Pyrococcus furiosus) is used widely because of its higher fidelity when copying DNA.

3. Primers- a short sequence of nucleotides that provides an initiation point for DNA synthesis.

4. dNTPs(deoxynucleotides triphosphates)- single units of the bases A, T, G, and C, which are essentially "building blocks" for new DNA strands.

Steps in PCR:

1. Denaturation (96°): Heat the reaction strongly to separate, or denature, the DNA strands. This provides a single-stranded template for the next step.

2. Annealing (55°C-65°C): Cool the reaction so the primers can bind to their complementary sequences on the single-stranded template DNA.

3. The extension (72°): Raise the reaction temperatures so Taq polymerase extends the primers, synthesizing new strands of DNA.

0 Dislike
Follow 2

Please Enter a comment


Other Lessons for You

Lets Study Basics of Molecular Inheritance:
Difference between RNA and DNA 2’ oh group in aldopentose U instead of T Most RNAs carry out their functions as single strands Structurally and functionally more diverse suited to a diverse...

Arpita D. | 2 days ago

0 0

Class 11 Biology: Biomolecules- Carbohydrates
CARBOHYDRATES Compounds of C, H, O Also called saccharides (sugars) Biomicromolecules- monosaccharides, derived monosaccharides, olgiosaccharides) Biomacromolecues- polysacharadies MONOSUGARS/MONOSACCHARIDES General...

Ravi Singh | 27 Feb

0 0

The site of Photosynthesis:- NEET/AIIMS/JIPMER
The site of Photosynthesis Photosynthesis takes place in Mesophyll cells inside chloroplasts. It is a process which involves chemical reactions in which water and carbon dioxide are converted into energy...

Anmol Sharma | 10/10/2019

0 0

How to remember the nature of 12 pairs of cranial nerves?
Trick- Some Says Money Matters But My Brother Says Big Books Matter Most. Now know the initial letter of each word written in capital. And decode them as S stands for sensory, B stands for both(mixed)...

Monalisha M. | 29/08/2019

0 0

Advances in genome engineering
Genome engineering or gene editing is a technique where the DNA is added, removed, modified or replaced in the genome of a living organism. CRISPR–Cas9 genome-editing technique is more precise...

Biju G. | 29/08/2019

0 0

Looking for Class 12 Tuition ?

Find best Class 12 Tuition in your locality on UrbanPro.

Are you a Tutor or Training Institute?

Join UrbanPro Today to find students near you

Looking for Class 12 Tuition Classes?

Find best tutors for Class 12 Tuition Classes by posting a requirement.

  • Post a learning requirement
  • Get customized responses
  • Compare and select the best

Looking for Class 12 Tuition Classes?

Find best Class 12 Tuition Classes in your locality on UrbanPro

Post your learning requirement is India's largest network of most trusted tutors and institutes. Over 25 lakh students rely on, to fulfill their learning requirements across 1,000+ categories. Using, parents, and students can compare multiple Tutors and Institutes and choose the one that best suits their requirements. More than 6.5 lakh verified Tutors and Institutes are helping millions of students every day and growing their tutoring business on Whether you are looking for a tutor to learn mathematics, a German language trainer to brush up your German language skills or an institute to upgrade your IT skills, we have got the best selection of Tutors and Training Institutes for you. Read more