Acetylation and Transcription

The core histones, particularly H3 and H4, are reversibly acetylated at the epsillon-amino groups of lysines in the N-terminal ends. It has been postulated that core histone acetylation of may be involved in the process of transcriptional activation because acetylation of the lysine residues neutralizes their positive charge and would certainly reduce their net positive charges and would presumably reduce the interaction of the core histone tails with DNA

Topics Covered

Basics of DNA replication and Transcription. Transcriptional activation and repression
Eukaryotic Gene regulation and Chromatin Structure, CpG Islands, Hypermethylation, Experimental Procedures for Gene Cloning, X-ray structure of Chromatin, Higher order structure of Chromatin

Who should attend

Undergraduate and Post Graduate Students of Biotechnology, Zoology, Genetics Molecular Biology and Botany

Pre-requisites

Chemistry of DNA and RNA, Prokaryotic gene regulaion, Operon Concept, Histone and Non histone proteins. Basic Cytogenetic principles, Cell Biology and Functional Molecular Biology. Basics of Biochemistry and Enzymology

What you need to bring

Nothing in Particular, Laptop wiih Internet connectivity, Possibly wifi, Digital Pen, Skype connection or G talk If Ina class setting, an exercise book, color pencils, good quality pens sharpners and erasers

Key Takeaways

The student will be aware of the recent exciting discoveries in Molecular biology, Biotechnology, Genetics and Cell Biology. Experimental and inferential questions will follow each sessions, making the student more confident to answer viva voce, critical thinking, analytical knowledge, and state of the art methodologies.. If possible a visit to a sophisticated laboratory may be undertaken as a bonus