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Bioinformatics

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Sanjeeva Reddy Nagar, Hyderabad

Course ID: 28363

Sanjeeva Reddy Nagar, Hyderabad

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₹ 40,000

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Zahoor

Ph.D Biotech

About Zahoor

Teaching Experience : 7 years (Till to date)

Teaching Chemical Engineering and Biotechnology subjects
for MTech (Biotechnology) and BTech (Biotechnology) students.

Research : 3 years
Industry Experience : More than 3 years of experience in guiding scientists from a leading pharma company on patents, IPR etc.
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About the Course

Subjects Taught/Handled

A) BTech Biotechnology Subjects

Chemical Engineering

1) Heat Transfer in Bioprocess,
2) Bioprocess Engineering I ,
3) Bioprocess Engineering I
4) Instrumentation & Process Control,
5) Biochemical Reaction Engineering I,
6) Biochemical Reaction Engineering II,
7) Mass Transfer & Separation,


Biotechnology
1) Biopharmaceutical Technology
2) Molecular Pathogenesis,
3) Protein Engineering,
4) Genomics & Proteomics,
5) Genetics,
6) Environmental Studies,
7) Biodiversity & Intellectual Property Rights,
8) Cell Biology,
9) Microbiology.
10) Nanobiotechnology,
11) Bioinformatics
12) Computational Molecular Biology
13) Immunology
14) Clinical Trials and regulatory Affairs

B) MTech Biotechnology Subjects

Chemical Engineering

1) Microbial Engineering
2) Enzyme Engineering
3)Bioreactor Engineering

Biotechnology

1. Genetic Engineering

Laboratories Handled

1. Bioinformatics Lab
2. Microbiology Lab
3. Biochemistry Lab
4. Plant Tissue Culture Lab
5. Bioprocess Engineering Lab
6. Molecular Biology & Genetic Engineering Lab

ACHEIVEMENTS

? Orgnanising Committee Memeber National conference on Advancements and Innovations in Engineering, Science and management 2014
? Review Panel in World Journal of Engineering Science
? Biography enlisted in Who’s Who in the World 2014
? Ratified as Assistant Professor of Biotechnology by Jawaharlal Nehru Technological University, Hyderabad.A.P. INDIA (2011)
? Selected for DBT sponsored Biotech Industrial Training Programme (BITP) BCIL 2010-2011., Govt of India.

PROJECTS

PhD Title (pursuing): Isolation, purification and characterisation of chitinolytic and chitosanalytic enzymes from Malabranchea chrysosporoidea

Topics Covered

Major Bioinformatics Resources: NCBI, EBI, ExPASy, RCSB:
• The knowledge of various databases and bioinformatics tools available at these resources, organization of databases: data contents and formats, purpose and utility in Life Sciences
Open access bibliographic resources and literature databases:
• Open access bibliographic resources related to Life Sciences viz., PubMed, BioMed Central, Public Library of Sciences (PloS)
Sequence databases:
• Formats, querying and retrieval
• Nucleic acid sequence databases: GenBank, EMBL, DDBJ;
• Protein sequence databases: Uniprot-KB: SWISS-PROT, TrEMBL, PIR-PSD
• Repositories for high throughput genomic sequences: EST, STS GSS, etc.;
• Genome Databases at NCBI, EBI, TIGR, SANGER
• Viral Genomes
• Archeal and Bacterial Genomes;
• Eukaryotic genomes with special reference to model organisms (Yeast, Drosophila, C. elegans, Rat, Mouse, Human, plants such as Arabidopsis thaliana, Rice, etc.)
3D Structure Database: PDB, NDB
• Chemical Structure database: Pubchem
• Gene Expression database: GEO, SAGE
Derived Databases:
• Knowledge of the following databases with respect to: basic concept of derived databases, sources of primary data and basic principles of the method for deriving the secondary data, organization of data, contents and formats of database entries, identification of patterns in given sequences and interpretation of the same
• Sequence: InterPro, Prosite, Pfam, ProDom, Gene Ontology
• Structure classification database: CATH,SCOP, FSSP
• Protein-Protein interaction database: STRING
Compilation of resources:
• NAR Database and Web server Issues and other resources published in Bioinformatics related journals
Sequence Analysis:

File formats:
• Various file formats for bio-molecular sequences: GenBank, FASTA, GCG, MSF etc
Basic concepts:
• Sequence similarity, identity and homology, definitions of homologues, orthologues, paralogues
Scoring matrices:
• basic concept of a scoring matrix, Matrices for nucleic acid and proteins sequences, PAM and BLOSUM series, principles based on which these matrices are derived
Pairwise sequence alignments:
• Basic concepts of sequence alignment: local and global alignments, Needleman and Wunsch, Smith and Waterman algorithms for pairwise alignments, gap penalties, use of pairwise alignments for analysis of Nucleic acid and protein sequences and interpretation of results.
Multiple sequence alignments (MSA):
• The need for MSA, basic concepts of various approaches for MSA (e.g. progressive, hierarchical etc.). Algorithm of CLUSTALW and PileUp and their application for sequence analysis (including interpretation of results), concept of dandrogram and its interpretation
Database Searches:
• Keyword-based searches using tools like ENTREZ and SRS
• Sequence-based searches: BLAST and FASTA
Sequence patterns and profiles:
• Basic concept and definition of sequence patterns, motifs and profiles, various types of pattern representations viz. consensus, regular expression (Prosite-type) and sequence profiles; profile-based database searches using PSI-BLAST, analysis and interpretation of profile-based searches
Taxonomy and phylogeny:
• Basic concepts in systematics, taxonomy and phylogeny; molecular evolution; nature of data used in Taxonomy and Phylogeny, Definition and description of phylogenetic trees and various types of trees
Protein and nucleic acid properties:
• Computation of various parameters using proteomics tools at the ExPASy server and EMBOSS
Comparative genomics:
• Basic concepts and applications, whole genome alignments: understanding significance. Artemis as an example
Sequence analysis: Scoring matrices:
• Detailed method of derivation of the PAM and BLOSUM matrices
Pairwise sequence alignments:
• Needleman and Wuncsh, Smith and Waterman algorithms and their implementation
Multiple sequence alignments (MSA):

Use of HMM-based Algorithm for MSA (e.g. SAM method)
Sequence patterns and profiles:

• Repeats: Tandem and Interspersed repeats, repeat finding, Motifs, consensus, position weight matrices
• Algorithms for derivation of and searching sequence patterns: MEME, PHI-BLAST, SCanProsite and PRATT
• Algorithms for generation of sequence profiles: Profile Analysis method of Gribskov, HMMer, PSI-BLAST
Protein and nucleic acid properties: e.g. Proteomics tools at the ExPASy server and EMBOSSTaxonomy and phylogeny:
• Phylogenetic analysis algorithms such as maximum Parsimony, UPGMA, Transformed Distance, Neighbors-Relation, Neighbor-Joining, Probabilistic models and associated algorithms such as Probabilistic models of evolution and maximum likelihood algorithm, Bootstrapping methods, use of tools such as Phylip, Mega, PAUP
• Analysis of regulatory RNAs: Databases and tools

Who should attend

BTech(Biotech) ., MTech (Biotech).,(Ph.D)(Biotech)
M.Sc(Biotech) & M.Sc (Life Sciences)

Pre-requisites

Basic Biotechnology concepts

What you need to bring

Latest PC configuration

Key Takeaways

Strong conceptual understanding and handson practice

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