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How to identify an unknown sample whether it is a DNA sample or RNA sample? And by which method can you determine its concentration?

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MS SQL SERVER DBA Trainer

You can getting your answer by given below details-- Absorbance Methods The most common technique to determine DNA yield and purity is measurement of absorbance. Although it could be argued that fluorescence measurement is easier, absorbance measurement is simple, and requires commonly available...
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You can getting your answer by given below details-- Absorbance Methods The most common technique to determine DNA yield and purity is measurement of absorbance. Although it could be argued that fluorescence measurement is easier, absorbance measurement is simple, and requires commonly available laboratory equipment. All that is needed for the absorbance method is a spectrophotometer equipped with a UV lamp, UV-transparent cuvettes (depending on the instrument) and a solution of purified DNA. Absorbance readings are performed at 260nm (A260) where DNA absorbs light most strongly, and the number generated allows one to estimate the concentration of the solution. To ensure the numbers are useful, the A260 reading should be within the instrument's linear range (generally 0.1–1.0). DNA concentration is estimated by measuring the absorbance at 260nm, adjusting the A260 measurement for turbidity (measured by absorbance at 320nm), multiplying by the dilution factor, and using the relationship that an A260 of 1.0 = 50µg/ml pure dsDNA. Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml Total yield is obtained by multiplying the DNA concentration by the final total purified sample volume. DNA yield (µg) = DNA concentration × total sample volume (ml) However, DNA is not the only molecule that can absorb UV light at 260nm. Since RNA also has a great absorbance at 260nm, and the aromatic amino acids present in protein absorb at 280nm, both contaminants, if present in the DNA solution, will contribute to the total measurement at 260nm. Additionally, the presence of guanidine will lead to higher 260nm absorbance. This means that if the A260 number is used for calculation of yield, the DNA quantity may be overestimated. To evaluate DNA purity, measure absorbance from 230nm to 320nm to detect other possible contaminants. The most common purity calculation is the ratio of the absorbance at 260nm divided by the reading at 280nm. Good-quality DNA will have an A260/A280 ratio of 1.7–2.0. A reading of 1.6 does not render the DNA unsuitable for any application, but lower ratios indicate more contaminants are present. The ratio can be calculated after correcting for turbidity (absorbance at 320nm). DNA purity (A260/A280) = (A260 reading – A320 reading) ÷ (A280 reading – A320 reading) read less
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Biology Rockstar

we can determine spectrophotometrically whether a given sample is DNA or RNA.... the absorbance given by RNA at 280 nm is much larger than the absorbance shown by the same amount of DNA at the same wavelength. Concentration can also be determined spectrophotometrically.
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Biotechnologist

Samples can be measured under UV 260nm , if the reading 260/280 is between 1.6-1.8 it is DNA, if it is greater than 1.9 then it is RNA. Agarose gel electrophoresis is also one method to identify, genomic DNA has a high molecular weight and mobility is less. where as RNA has a low molecular weight...
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Samples can be measured under UV 260nm , if the reading 260/280 is between 1.6-1.8 it is DNA, if it is greater than 1.9 then it is RNA. Agarose gel electrophoresis is also one method to identify, genomic DNA has a high molecular weight and mobility is less. where as RNA has a low molecular weight and it moves faster. read less
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Biotechnology professional

High throughput detection kitsdetection are also available for on spot
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Trainer

spectroscopy
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Biologist

DNA and RNA is identified by three way 1. by biochemical analysis 2. by molecular biology technique 3. biochemical technique.
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Teacher

Quantitative & qualitative- colorimetric (DNA by DPA method, RNa by Orcinol method )or also by spectrophotometric method can be conducted.
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Molecular Transformationist

Assuming the DNA / RNA is very much limited in quantity, UV spec by a Nano Drop equipment will be the best option. If the available quantity of the sample is more, running a Gel Electrophoresis (Agarose or Poly-Acrylamide) will give the approximate concentration and quality of the sample. If...
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Assuming the DNA / RNA is very much limited in quantity, UV spec by a Nano Drop equipment will be the best option. If the available quantity of the sample is more, running a Gel Electrophoresis (Agarose or Poly-Acrylamide) will give the approximate concentration and quality of the sample. If there is some idea about the origin and speculated identity, then a PCR can be done using specific primers. read less
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teacher is the builder of future of country

Identification by spectroscopy . by colligative osmotic pressure property
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Coaching

The most common technique to determine DNA yield and purity is measurement of absorbance. All that is needed for the absorbance method is a spectrophotometer equipped with a UV lamp, UV-transparent cuvettes (depending on the instrument) and a solution of purified DNA. DNA concentration is estimated...
read more
The most common technique to determine DNA yield and purity is measurement of absorbance. All that is needed for the absorbance method is a spectrophotometer equipped with a UV lamp, UV-transparent cuvettes (depending on the instrument) and a solution of purified DNA. DNA concentration is estimated by measuring the absorbance at 260nm, adjusting the A260 measurement for turbidity (measured by absorbance at 320nm), multiplying by the dilution factor, and using the relationship that an A260 of 1.0 = 50µg/ml pure dsDNA. Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml Total yield is obtained by multiplying the DNA concentration by the final total purified sample volume. DNA yield (µg) = DNA concentration × total sample volume (ml) read less
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